Mal-PEG2-NHS ester

Seo, Jeong Hyun, et al. Biomaterials 32.23 (2011): 5478-5488.

Glass substrates were functionalized using a binary mixture of acrylates and thiol-terminated methoxysilanes. In the subsequent hydrogel photopatterning step, the acrylate moiety was used to anchor the hydrogel microwells to the glass substrate. Importantly, the glass attachment sites within the microwells contain thiol groups that can be activated with heterobifunctional crosslinkers to covalently immobilize proteins. After incubation with fluorescently labeled avidin, microwells fabricated on the hybrid acrylate/thiol silane layer emitted approximately 6 times more fluorescence than microwells fabricated on acrylate silane alone.
Smaller and larger masses observed in SIMS analysis of hybrid silane surfaces incubated in MAL-PEG2-NHS ester linker and avidin. Analysis of the surface treated with the linker (showing the presence of acrylate and thiol groups at m / z 32, 33, 119, and 179, and the linker at m / z 256 (C 11 H 16 N 2 O 5 -) and 270 (C 12 H 18 N 2 O 5 -). The surface incubated with avidin shows the presence of peaks associated with acrylate and thiol groups, as well as the linker and avidin (bottom spectrum). The negative ions at m / z 91 (Phe -) and 107 (Tyr -) were attributed to the peaks originating from the amino acid residues of avidin.